Replacement for much used GCG programs

 

 

Exchanging sequences between packages

 

In WWW2GCG

In wEMBOSS

getseq

newseq

fromfasta , tofasta

fromembl , toembl

from* , to*

seqret

(any EMBOSS program that reads/writes sequences)

 

 

Sequence editing, manipulation and display

 

In WWW2GCG

In wEMBOSS

fetch

seqret

seqed

command delete

command insert

no complete solution yet

cutseq

pasteseq

lineup

no good solution yet

assemble

union

shuffle

shuffleseq

reverse

revseq

chopup

no more needed as EMBOSS reads any format

publish

showseq, prettyseq

 

 

Translation

 

In WWW2GCG

In wEMBOSS

translate

transeq

 

Sequence comparison and alignment

 

In WWW2GCG

In wEMBOSS

compare+dotplot (default (window stringency))

compare+dotplot (word=n)

dotmatcher

dottup

gap

needle, stretcher (for long sequences)

bestfit

water, matcher (for long sequences)

pileup, clustal

clustal

pretty

cons, showalign

 

 

Keyword-based databank searching

In WWW2GCG

In wEMBOSS

names

whichdb

indexsearch

indexsearch

stringsearch (mode A)

stringsearch (mode B)

textsearch

no good solution yet but advantageously replaceable by indexsearch

 


Sequence-based databank searching

In WWW2GCG

In wEMBOSS

blast

blast

psiblast

psiblast

fasta

fasta

 

 

Patterns and gene finding

 

In WWW2GCG

In wEMBOSS

findpatterns

fuzznuc, fuzztrans, fuzzprot

NB: uses PROSITE syntax (not GCG) to define pattern

 

motifs

patmatmotifs

NB: ps_scan searches also PROSITE profiles

codonpreference

syco, wobble

 

 

Phylogeny

 

In WWW2GCG

In wEMBOSS

distances+growtree

clustalnj or ednadist/eprotdist+eneighbor

 

 

Mapping

 

In WWW2GCG

In wEMBOSS

map

- with option "Find translationally silent potential restriction sites"

remap, restrict

silent

Mapsort

mapsort+plasmidmap

restrict

cirdna (only partial solution : input file with Tick

positions must be created "manually")

 

 

Protein analysis

 

In WWW2GCG

In wEMBOSS

pepplot, peptidestructure+plotstructure

garnier, pepinfo, octanol, pepwindow

 

 

Primer selection

 

In WWW2GCG

In wEMBOSS

prime

eprimer3 (=Primer3)

primepair, melttemp

no good solution yet

 

 

DNA fragment assembly

 

gelstart

gelenter

gelmerge

gelassemble

gelview

geldiassemble

no good solution yet in In wEMBOSS but well replaced by STADEN pregap4+gap4, accessible through a X-window interface. Contact BEN for more information